YAC subclone contig assembly by serial interspersed repetitive sequence (IRS)-PCR product hybridizations
نویسندگان
چکیده
منابع مشابه
YAC subclone contig assembly by serial interspersed repetitive sequence (IRS)-PCR product hybridizations.
Yeast artificial chromosomes (YACs) are presently the most effective means of cloning large contiguous regions of genomic DNA (1). However, performing a detailed molecular analysis of YAC clone insert DNA is constrained by inherent physical and technical limitations. Typically, an extended analysis of YAC DNA is facilitated by subcloning YAC insert DNA into lambda bacteriophage or cosmid vector...
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Interspersed repetitive element (IRE)-PCR is a useful method for identification of novel human or mouse sequence tagged sites (STSs) from contigs of genomic clones. We describe the use of IRE-PCR with mouse B1 repetitive element primers to generate novel, PCR amplifiable, simple sequence length polymorphisms (SSLPs) from yeast artificial chromosome (YAC) clones containing regions of mouse chrom...
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Using two recently developed techniques, IRS-PCR YAC walking and IRS-PCR genotyping, a framework-integrated physical and genetic map of the mouse genome was constructed. The map consists of 821 contigs, containing 7746 YAC clones originating from three different YAC libraries. Three hundred eighty of the contigs have been anchored to the genetic map. Approximately 16% of the physical length of ...
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A plethora of algorithmic assemblers have been proposed for the de novo assembly of genomes, however, no individual assembler guarantees the optimal assembly for diverse species. Optimizing various parameters in an assembler is often performed in order to generate the most optimal assembly. However, few efforts have been pursued to take advantage of multiple assemblies to yield an assembly of h...
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Due to its unique biology of partial sex linkage and high recombination rates, the pseudoautosomal region (PAR1) on both X and Y chromosomes has attracted considerable interest. In addition, an extremely high level of YAC instability has been observed in this region. We have derived 82 YAC clones from six different YAC libraries mapping to this 2.6-Mb region. Of these a subset of 22 YACs was an...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1993
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/21.22.5275